| Gene | Tmem129 |
| Chromosome | 5 |
| Deletion Start | 33,657,824 |
| Deletion End | 33,654,333 |
| Deletion Size | 3,492 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,657,783 | 33,657,802 | CGTTGGCCTACCTGGTCTTC |
| TUP | 33,657,756 | 33,657,778 | TGTGCTTCGTGTTCACGCCCAAC |
| TUR | 33,657,708 | 33,657,726 | CCAGCCCGACAACAGATTC |
| TDF | 33,654,894 | 33,654,915 | CAAGGGCAGATGGTCTCAGTTC |
| TDP | 33,654,868 | 33,654,891 | CAAGCCATGGTTCTTGCAGGCTGA |
| TDR | 33,654,808 | 33,654,825 | TGGCTGCACTTCGGATTG |
PCR Primers
| Primer | Sequence |
| SU | CTTCGGGTCAACCCAACAG |
| SD | GCATCCTATGTGGCATATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 311 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10468A-B5 | VGB6 | C57BL/6NTac | |
| 10468A-C1 | VGB6 | C57BL/6NTac | |
| 10468A-D10 | VGB6 | C57BL/6NTac | |
| 10468A-D4 | VGB6 | C57BL/6NTac | |
| 10468A-E2 | VGB6 | C57BL/6NTac | |
| 10468A-C7 | VGB6 | C57BL/6NTac | |
