| Gene | Gpr153 |
| Chromosome | 4 |
| Deletion Start | 152,279,109 |
| Deletion End | 152,283,589 |
| Deletion Size | 4,481 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 152,279,741 | 152,279,760 | GGAGGCTACAGTGTCAGTGG |
| TUP | 152,279,767 | 152,279,789 | ATGGTCTGGGCCCTCTGTGGTCC |
| TUR | 152,279,815 | 152,279,837 | GGAGCAGGGCAGAATAGAGAATC |
| TDF | 152,282,701 | 152,282,724 | CCATTGGTGTCTTGTGCTGTAAAC |
| TDP | 152,282,725 | 152,282,750 | CTTTCCATGAGCCTTCGCTCCAACCG |
| TDR | 152,282,758 | 152,282,778 | GAAGCTGGGTGTTGGCTGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTTAGGCCTTCAACCCAGTG |
| SD | CTTCCTGGGAGATGCCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10470C-C3 | VGB6 | C57BL/6NTac | |
| 10470C-G8 | VGB6 | C57BL/6NTac | |
