| Gene | Gpx8 |
| Chromosome | 13 |
| Deletion Start | 113,046,372 |
| Deletion End | 113,043,115 |
| Deletion Size | 3,258 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,045,637 | 113,045,659 | GCTTCACAGACAAGAGTTACCAG |
| TUP | 113,045,608 | 113,045,635 | CTCTCAGGGAGCTACACAAGGAGTTCGG |
| TUR | 113,045,550 | 113,045,568 | GGGCTCCGATTCTCCAAAC |
| TDF | 113,043,737 | 113,043,760 | TCAGAGTTAGCCTGAGCTATGTAG |
| TDP | 113,043,712 | 113,043,736 | TGAATTTGAGGCCAGCCTGGGCTAC |
| TDR | 113,043,637 | 113,043,654 | TGCTGGGACTCACAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TGCTGAGCAGCCCGATGACC |
| SD | TTAATCCTAGAGAAAGTTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 445 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10471A-D10 | VGB6 | C57BL/6NTac | |
| 10471A-D12 | VGB6 | C57BL/6NTac | |
| 10471A-F6 | VGB6 | C57BL/6NTac | |
| 10471A-F9 | VGB6 | C57BL/6NTac | |
| 10471A-H10 | VGB6 | C57BL/6NTac | |
| 10471A-C12 | VGB6 | C57BL/6NTac | |
