| Gene | Wnt8a |
| Chromosome | 18 |
| Deletion Start | 34,542,354 |
| Deletion End | 34,547,839 |
| Deletion Size | 5,486 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,542,396 | 34,542,414 | GCTGCGGGCATGTGCTATC |
| TUP | 34,542,415 | 34,542,435 | CAGCCCTGGGTGCTTCTGCCT |
| TUR | 34,542,441 | 34,542,462 | GGACTAAAGGCTGGAGAGTGAC |
| TDF | 34,546,842 | 34,546,863 | GCTTCCGTCATCTTCTTAGCAC |
| TDP | 34,546,864 | 34,546,886 | TTGGCCAGCTCCTGGTCCTAACC |
| TDR | 34,546,922 | 34,546,940 | GGGCACTCCTAACCCTGTC |
PCR Primers
| Primer | Sequence |
| SU | GGTAGGAGACCTGCTTCAGC |
| SD | CAGAATTCCTTTCCTTTAACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 302 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10481B-F10 | VGB6 | C57BL/6NTac | |
| 10481B-H3 | VGB6 | C57BL/6NTac | |
