| Gene | 9230104L09Rik |
| Chromosome | 2 |
| Deletion Start | 148,850,877 |
| Deletion End | 148,846,893 |
| Deletion Size | 3,985 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,850,833 | 148,850,857 | ACTGGCTCTCTCTGGTATCTATGTC |
| TUP | 148,850,804 | 148,850,827 | AGCTCAGGCATGGTGTTCGGAAGA |
| TUR | 148,850,734 | 148,850,755 | GTGGAATGCACTCAAAGCAAAC |
| TDF | 148,847,645 | 148,847,668 | CCATAGCTTCAGAATGCTCATGTC |
| TDP | 148,847,621 | 148,847,643 | TCTTTGGTCCTGCATGCTGAGCC |
| TDR | 148,847,569 | 148,847,591 | CCTGAATCCAAGGGAGGAGAATG |
PCR Primers
| Primer | Sequence |
| SU | GAGGGAGGTGGTACCAAGG |
| SD | AACTCCACTAAGTGCTCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 287 bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 332 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10482A-A3 | VGB6 | C57BL/6NTac | |
| 10482A-A4 | VGB6 | C57BL/6NTac | |
| 10482A-A8 | VGB6 | C57BL/6NTac | |
| 10482A-A10 | VGB6 | C57BL/6NTac | |
| 10482A-C1 | VGB6 | C57BL/6NTac | |
| 10482A-F4 | VGB6 | C57BL/6NTac | |
