| Gene | Tmed3 |
| Chromosome | 9 |
| Deletion Start | 89,704,922 |
| Deletion End | 89,699,724 |
| Deletion Size | 5,199 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,704,806 | 89,704,824 | CTTCGAGCTGCCGGACAAC |
| TUP | 89,704,766 | 89,704,789 | TCACGCCCTGCTCCACCTCTTCGT |
| TUR | 89,704,736 | 89,704,754 | AGGGCCGCACCTGGTAATC |
| TDF | 89,700,034 | 89,700,055 | CAGTAAACCTTGGCTGCTGGAT |
| TDP | 89,700,004 | 89,700,031 | TGCCCTGCCTGACTTAGGGTTAGCAACT |
| TDR | 89,699,976 | 89,699,998 | CCATCTAGGCAGGAAGAAAACAG |
PCR Primers
| Primer | Sequence |
| SU | ACTGAGAAGTACCCGAGTCC |
| SD | GCCTCCCGTAGTGACCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 362 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10488A-C8 | VGB6 | C57BL/6NTac | |
