| Gene | C1ql1 |
| Chromosome | 11 |
| Deletion Start | 102,946,458 |
| Deletion End | 102,939,707 |
| Deletion Size | 6,752 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,945,574 | 102,945,591 | TCTAGGGCGCACGAACTG |
| TUP | 102,945,539 | 102,945,562 | TTTGGCCCGTGGGAGACTGATTCA |
| TUR | 102,945,498 | 102,945,515 | TCGGCGTCAGCTTGAATC |
| TDF | 102,939,854 | 102,939,877 | GAACTACGACTATGCCAGCAACAG |
| TDP | 102,939,829 | 102,939,851 | TGATCCTGCATCTGGACGCAGGG |
| TDR | 102,939,791 | 102,939,810 | TGTGCTTTGCCTCCATCCAG |
PCR Primers
| Primer | Sequence |
| SU | GCCGGACCGCCCTTGAGC |
| SD | ACCAGGAAGGCAACCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 280 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 314 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10500A-A11 | VGB6 | C57BL/6NTac | |
| 10500B-H5 | VGB6 | C57BL/6NTac | |
| 10500C-B9 | VGB6 | C57BL/6NTac | |
| 10500C-C5 | VGB6 | C57BL/6NTac | |
| 10500C-H12 | VGB6 | C57BL/6NTac | |
| 10500D-E3 | VGB6 | C57BL/6NTac | |
| 10500C-F4 | VGB6 | C57BL/6NTac | |
