| Gene | Krt16 |
| Chromosome | 11 |
| Deletion Start | 100,248,887 |
| Deletion End | 100,246,214 |
| Deletion Size | 2,674 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,248,548 | 100,248,567 | CCTGGTGGGCAGTGAGAAAG |
| TUP | 100,260,682 | 100,260,706 | CCATGCAGAACCTCAATGACCGCCT |
| TUR | 100,248,468 | 100,248,488 | CAGGTCTCTGTTGGCCTCTTC |
| TDF | 100,246,602 | 100,246,624 | TGGCGAGAATATCCAGTGAGTTG |
| TDP | 100,246,575 | 100,246,600 | CCCTGAGTCCTTGCTCACAGTACCTC |
| TDR | 100,246,554 | 100,246,572 | GTGCAGGGAGCAGCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CCACACCCACAGATCTGGGC |
| SD | TAGACATCACACAAGTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 380 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 239 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10501A-E2 | VGB6 | C57BL/6NTac | |
| 10501A-F2 | VGB6 | C57BL/6NTac | |
| 10501A-A5 | VGB6 | C57BL/6NTac | |
| 10501B-H1 | VGB6 | C57BL/6NTac | |
