| Gene | Abpg |
| Chromosome | 7 |
| Deletion Start | 33,944,961 |
| Deletion End | 33,943,166 |
| Deletion Size | 1,796 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,944,411 | 33,944,432 | GCCAGAACTTACGGAGCAATAC |
| TUP | 33,944,382 | 33,944,408 | ACTCTAAGGAGGACCTTCCTGCATGGT |
| TUR | 33,944,339 | 33,944,359 | AGGCCACCCTTTCAGCTACAG |
| TDF | 33,943,438 | 33,943,457 | GCTTCCTCCTGAAGGCTGTG |
| TDP | 31,359,282 | 31,359,303 | TGGGCTCCAGTTCCCTTGAGGC |
| TDR | 33,943,383 | 33,943,406 | GCAATGATTCAGTTCTCCAGCAAC |
PCR Primers
| Primer | Sequence |
| SU | AGGAACAAACAACTCTCCAG |
| SD | GGATGAACTGGACAGTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10506A-A11 | VGB6 | C57BL/6NTac | |
| 10506A-G5 | VGB6 | C57BL/6NTac | |
| 10506B-B4 | VGB6 | C57BL/6NTac | |
| 10506B-A4 | VGB6 | C57BL/6NTac | |
