| Gene | Svs2 |
| Chromosome | 2 |
| Deletion Start | 164,238,240 |
| Deletion End | 164,236,858 |
| Deletion Size | 1,383 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,238,204 | 164,238,225 | GTCCTTTCTCTGCTCCTCATTC |
| TUP | 164,238,174 | 164,238,203 | TGGAAAGACAGTCAGCTGTGGTTGGACAAT |
| TUR | 164,238,143 | 164,238,166 | CCTCTATCCACCATCCCTATACAC |
| TDF | 164,237,097 | 164,237,118 | GGTATGTCACAAGTGCGGAAAC |
| TDP | 164,237,060 | 164,237,089 | TGACGATGACCTCTCAGTACAACAGAAGTC |
| TDR | 164,236,996 | 164,237,016 | TTGTCCATATTGCCGCTGTTG |
PCR Primers
| Primer | Sequence |
| SU | GGATGCGATAAAGTCAGAGG |
| SD | TTAATGTAGTAATACAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 412 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | 371 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10507A-D7 | VGB6 | C57BL/6NTac | |
| 10507D-E1 | VGB6 | C57BL/6NTac | |
