| Gene | Nmrk2 |
| Chromosome | 10 |
| Deletion Start | 81,201,973 |
| Deletion End | 81,199,340 |
| Deletion Size | 2,634 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,201,356 | 81,201,376 | GACAGGCAACTTAGGGCAGAG |
| TUP | 81,201,322 | 81,201,349 | ATTTCCAGAATGGCTGAGGATGACACGA |
| TUR | 81,201,286 | 81,201,305 | GGCAGTAGGTCACAGCAATC |
| TDF | 81,199,670 | 81,199,690 | CTGATCCTCCTGGCCTGTTTG |
| TDP | 81,199,646 | 81,199,668 | TGGTCACGTGTGGCCCATGTACC |
| TDR | 81,199,622 | 81,199,644 | GCTCCATCTCCCGTCTATACTTC |
PCR Primers
| Primer | Sequence |
| SU | TATTTGCAGCCTATGAAACAGG |
| SD | ACAAGGTTACCCACAGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 318 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10509A-A12 | VGB6 | C57BL/6NTac | |
| 10509A-B6 | VGB6 | C57BL/6NTac | |
| 10509A-D8 | VGB6 | C57BL/6NTac | |
| 10509A-F5 | VGB6 | C57BL/6NTac | |
| 10509A-G11 | VGB6 | C57BL/6NTac | |
