10511   4933403G14Rik     Status: ES cell colonies screened / QC positives

Gene4933403G14Rik
Chromosome8
Deletion Start125,669,854
Deletion End125,672,545
Deletion Size2,692
Genome Build38
MGI IDMGI:1921643
NCBI Gene ID74393
BAC ID453F12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,670,334125,670,354CCCTGCTTGTATCCTGGACTC
TUP125,670,358125,670,380AGCCCTGGAGGTGTCAGAGCCAA
TUR125,670,391125,670,408GTGCATGGCTTCGAGGTC
TDF125,672,254125,672,274CCAGGCAGATCCAGGTAGTTC
TDP125,672,277125,672,305AAGTCATGAGGAAAGGACGGGACAGCTCC
TDR125,672,342125,672,362CCTTGGTGACTGGCTCTTCTC

PCR Primers

PrimerSequence
SUAAGACGTAGGACCTGCGGTG
SDTATGATTGGAGAGTATAGAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev373 bp-++
TUFTUR75 bp++-
TDFTDR109 bp++-
NeoFwdSD314 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10511A-B6VGB6C57BL/6NTac
10511A-D3VGB6C57BL/6NTac
10511A-H12VGB6C57BL/6NTac
10511A-H1VGB6C57BL/6NTac
10511A-E2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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