| Gene | 2310035K24Rik |
| Chromosome | 2 |
| Deletion Start | 131,211,301 |
| Deletion End | 131,213,172 |
| Deletion Size | 1,872 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,211,356 | 131,211,375 | TTTGCCGAGTGCTCTATTCC |
| TUP | 131,211,378 | 131,211,404 | TGTCTTTGGTGCTGAGAAATCACCCGA |
| TUR | 131,211,413 | 131,211,432 | CCTTTCTGCACCATGTGACC |
| TDF | 131,213,044 | 131,213,065 | TCAGGCCACTGGAATTGTAAAG |
| TDP | 131,213,069 | 131,213,097 | TCATTGTAGAGCATCCTGAAGCTGGAACC |
| TDR | 131,213,116 | 131,213,135 | GCGTGGTGGACACTGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCATTCAGCTGTGGTCTCTG |
| SD | AGAGGACAAGATCAGACCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10515A-A4 | VGB6 | C57BL/6NTac | |
| 10515A-B5 | VGB6 | C57BL/6NTac | |
| 10515A-G5 | VGB6 | C57BL/6NTac | |
| 10515A-E7 | VGB6 | C57BL/6NTac | |
| 10515A-F4 | VGB6 | C57BL/6NTac | |
