| Gene | Gsdma2 |
| Chromosome | 11 |
| Deletion Start | 98,666,043 |
| Deletion End | 98,657,988 |
| Deletion Size | 8,056 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,649,458 | 98,649,479 | TTCCTTACCACAGATCCAACAC |
| TUP | 98,649,506 | 98,649,534 | ATGCTGGATGTCAGAGTAGAGGGAGATGT |
| TUR | 98,649,549 | 98,649,571 | CACAGTCCCCTTTACCTTCATCA |
| TDF | 98,657,782 | 98,657,800 | AGCCTTCCCGGCCTTACTG |
| TDP | 98,657,801 | 98,657,828 | TGCTCTGTCTGAAACACTCCAAGATGGT |
| TDR | 98,657,850 | 98,657,870 | ACTGCCTCTGCCTCCTGACTG |
PCR Primers
| Primer | Sequence |
| SU | AGGGGAAGGCTGGCATCTTG |
| SD | GGAGTTGCTAAAGAGCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 17350 bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 386 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10517A-B3 | VGB6 | C57BL/6NTac | |
| 10517A-C7 | VGB6 | C57BL/6NTac | |
| 10517A-G2 | VGB6 | C57BL/6NTac | |
| 10517B-D9 | VGB6 | C57BL/6NTac | |
| 10517B-E11 | VGB6 | C57BL/6NTac | |
