| Gene | S100a3 |
| Chromosome | 3 |
| Deletion Start | 90,601,769 |
| Deletion End | 90,602,355 |
| Deletion Size | 587 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,601,806 | 90,601,825 | GCAGTAGCTGCCATCGTGTG |
| TUP | 90,601,830 | 90,601,851 | TTCCAGGAGTATGCAGGGCGCT |
| TUR | 90,601,884 | 90,601,906 | TCCTTCTGCAACAACTCCTTGAG |
| TDF | 90,602,160 | 90,602,177 | TCCTGACCCTGCCTTCTC |
| TDP | 90,602,181 | 90,602,204 | CCTTCCACAGAGTGAGTTCCGGGA |
| TDR | 90,602,236 | 90,602,259 | CACTTCGCAGTCTTTGTTGGTATC |
PCR Primers
| Primer | Sequence |
| SU | AATAAAAGACTGAGAATTCC |
| SD | AAGGCTGGCTACTGTCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10518A-B1 | VGB6 | C57BL/6NTac | |
| 10518A-B6 | VGB6 | C57BL/6NTac | |
| 10518A-C12 | VGB6 | C57BL/6NTac | |
| 10518A-F3 | VGB6 | C57BL/6NTac | |
| 10518A-F5 | VGB6 | C57BL/6NTac | |
| 10518A-F9 | VGB6 | C57BL/6NTac | |
