| Gene | Nrsn1 |
| Chromosome | 13 |
| Deletion Start | 25,262,388 |
| Deletion End | 25,253,353 |
| Deletion Size | 9,036 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,261,705 | 25,261,726 | TCCTGACTTCCTACCTCCACTG |
| TUP | 25,261,677 | 25,261,704 | TGCTTCCTGATTGTCCAAAGGGAGCAAG |
| TUR | 25,261,645 | 25,261,664 | GAAGGCTTGGCTCCTGACTG |
| TDF | 25,253,533 | 25,253,551 | GGCAGGGTGCCTACTGATG |
| TDP | 25,253,503 | 25,253,532 | TCTGTGTTTGCCAAGAGCTATTCCAAAGAA |
| TDR | 25,253,442 | 25,253,463 | GCTGGGTATGGGCTTTAATGTC |
PCR Primers
| Primer | Sequence |
| SU | ATAATTTAAATGACTGAAGC |
| SD | GATACACCAGAGACACTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 395 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10523A-H11 | VGB6 | C57BL/6NTac | |
| 10523A-E12 | VGB6 | C57BL/6NTac | |
| 10523B-D1 | VGB6 | C57BL/6NTac | |
| 10523B-F5 | VGB6 | C57BL/6NTac | |
| 10523B-F8 | VGB6 | C57BL/6NTac | |
| 10523B-G10 | VGB6 | C57BL/6NTac | |
