| Gene | Slc25a10 |
| Chromosome | 11 |
| Deletion Start | 120,491,970 |
| Deletion End | 120,498,223 |
| Deletion Size | 6,254 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,492,667 | 120,492,687 | TCATCTGCTTCCGCTTGTTTC |
| TUP | 120,492,689 | 120,492,714 | TTGCTATATTGGGATCAGCACCGTCC |
| TUR | 120,492,718 | 120,492,737 | GGGTCTCCTTCCTAGCTCAC |
| TDF | 120,497,354 | 120,497,375 | GGGAGGACCACTGACAGATTAC |
| TDP | 120,497,381 | 120,497,403 | CAGGGCGGATGTGCCACGTTTCT |
| TDR | 120,497,437 | 120,497,456 | ACTCGCCCTTGGAGTTCATC |
PCR Primers
| Primer | Sequence |
| SU | TGGGGGTGGTCATGGGTTAG |
| SD | TGCCAGGCAGGGCACGGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 387 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 351 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10524A-C10 | VGB6 | C57BL/6NTac | |
| 10524A-D4 | VGB6 | C57BL/6NTac | |
| 10524A-E5 | VGB6 | C57BL/6NTac | |
| 10524A-E12 | VGB6 | C57BL/6NTac | |
| 10524A-F1 | VGB6 | C57BL/6NTac | |
| 10524A-G7 | VGB6 | C57BL/6NTac | |
