| Gene | Gpihbp1 |
| Chromosome | 15 |
| Deletion Start | 75,596,682 |
| Deletion End | 75,598,161 |
| Deletion Size | 1,480 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,597,137 | 75,597,154 | TGGAAGCAGGGACAGAGG |
| TUP | 75,597,164 | 75,597,182 | TGAGGGCCCAGCTTCCTGC |
| TUR | 75,597,205 | 75,597,226 | ACCCAAGGGTGAGATGTAAGTG |
| TDF | 75,597,585 | 75,597,604 | GCAGCAAACCCTTCTGCATC |
| TDP | 75,597,605 | 75,597,625 | ACGCTCGTCTCCCACAGCGGA |
| TDR | 75,597,662 | 75,597,681 | TGTGGGCTCTTCCTCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTCCCTTTCCTCTCTATCC |
| SD | CACAGGCGATAGACAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 287 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10535A-A2 | VGB6 | C57BL/6NTac | |
| 10535A-B1 | VGB6 | C57BL/6NTac | |
| 10535A-B8 | VGB6 | C57BL/6NTac | |
| 10535A-H4 | VGB6 | C57BL/6NTac | |
