| Gene | Ttc22 |
| Chromosome | 4 |
| Deletion Start | 106,622,536 |
| Deletion End | 106,639,662 |
| Deletion Size | 17,127 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,623,353 | 106,623,377 | CCTCATCCACCTATCCTTCTAAACC |
| TUP | 106,623,383 | 106,623,407 | TTTGCCACTGCCTCAGCTTATACGA |
| TUR | 106,623,430 | 106,623,449 | AGCCAGGCAGGACACTTCAG |
| TDF | 106,638,962 | 106,638,982 | AGCTGCTGGCAGTGGATGAAG |
| TDP | 106,638,983 | 106,639,007 | CTGCGCTCAACCAGGCTCTGGTCTT |
| TDR | 106,639,022 | 106,639,042 | TGTCACCTAGCTCCAACTCAC |
PCR Primers
| Primer | Sequence |
| SU | GAAAGCGGAAACACCTACAG |
| SD | TGAGGAGCCTTGAGGCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 271 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10536D-G7 | VGB6 | C57BL/6NTac | |
| 10536D-E7 | VGB6 | C57BL/6NTac | |
| 10536E-D5 | VGB6 | C57BL/6NTac | |
| 10536E-E8 | VGB6 | C57BL/6NTac | |
| 10536E-F3 | VGB6 | C57BL/6NTac | |
