| Gene | Actrt2 |
| Chromosome | 4 |
| Deletion Start | 154,667,674 |
| Deletion End | 154,666,539 |
| Deletion Size | 1,136 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,667,419 | 154,667,437 | ACGAGCTGGGACGATGTGG |
| TUP | 154,667,383 | 154,667,412 | TCTGGAGACATCTCTTCGAATGGGAACTGG |
| TUR | 154,667,363 | 154,667,380 | TCGTTCGCAGGGCTTCAC |
| TDF | 154,666,886 | 154,666,905 | AGGCTCCTGAGGTCCTGTTC |
| TDP | 154,666,864 | 154,666,885 | TCACCAGACCAGTTGGGCACCC |
| TDR | 154,666,800 | 154,666,823 | TTCTGTATGTCAGCATCACACTTG |
PCR Primers
| Primer | Sequence |
| SU | CTCGGAGGATGGAGGGTTC |
| SD | GACAAGCATACACTCAAACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 307 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 276 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10537B-A8 | VGB6 | C57BL/6NTac | |
| 10537B-C2 | VGB6 | C57BL/6NTac | |
| 10537B-F10 | VGB6 | C57BL/6NTac | |
| 10537B-G6 | VGB6 | C57BL/6NTac | |
| 10537B-G10 | VGB6 | C57BL/6NTac | |
| 10537B-B3 | VGB6 | C57BL/6NTac | |
