10539   Prl3c1     Status: ES cell colonies screened / QC positives

GenePrl3c1
Chromosome13
Deletion Start27,196,709
Deletion End27,203,612
Deletion Size6,904
Genome Build38
MGI IDMGI:1351649
NCBI Gene ID27372
BAC ID434E10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF27,197,24127,197,260CTGTGGCTTTTGTTGCTGTC
TUP27,197,26127,197,284TGCTTGTAGAGCAGTGCAGGTTCA
TUR27,197,29927,197,322TGTTTCAGAGAAGGCAAAGACTTG
TDF27,202,99927,203,023GATAATATGCACCAGAACTGTGGAG
TDP27,203,02627,203,053TGTTCGTGTTTCTGAGGACTTTCTTGCA
TDR27,203,11027,203,134CCACTGTTGGAATTACAAGGAAAAC

PCR Primers

PrimerSequence
SUTATTCCCTGAAGGAAGAGAC
SDTTTATATTAATACTGATTAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev330 bp-++
TUFTUR82 bp++-
TDFTDR136 bp++-
NeoFwdSD431 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10539A-H5VGB6C57BL/6NTac
10539B-C7VGB6C57BL/6NTac
10539B-D10VGB6C57BL/6NTac
10539B-F4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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