| Gene | Rtp1 |
| Chromosome | 16 |
| Deletion Start | 23,429,182 |
| Deletion End | 23,431,678 |
| Deletion Size | 2,497 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,429,245 | 23,429,269 | TCTATAAAGTGTAGTTTGCCTCCTC |
| TUP | 23,429,271 | 23,429,299 | TCCCACTGACGAAGACATGTGTAAGAGTG |
| TUR | 23,429,306 | 23,429,327 | AAGACCTTCTTCCACTCACCTG |
| TDF | 23,430,908 | 23,430,929 | GGAAAGCAACTAGGGAGCAAAC |
| TDP | 23,430,933 | 23,430,958 | TACTAGATGCCCGATGGCAAGTCTGG |
| TDR | 23,430,966 | 23,430,985 | GAGTGCCCAAGGGACAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TGCCTCCAGGGTACAGCAAG |
| SD | GAAAAGGCAGAAGTTGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 434 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 395 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10544E-E11 | VGB6 | C57BL/6NTac | |
| 10544E-D12 | VGB6 | C57BL/6NTac | |
