| Gene | Gtdc2 |
| Chromosome | 9 |
| Deletion Start | 121,983,710 |
| Deletion End | 121,981,951 |
| Deletion Size | 1,760 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,983,090 | 121,983,114 | TGCCCACTTTGACCTCTATAAACTC |
| TUP | 121,983,061 | 121,983,083 | CCCAAGCAGCCGCTTCTACGTGC |
| TUR | 121,983,038 | 121,983,056 | GTCGGCCCAGTGTCTTGAG |
| TDF | 121,982,772 | 121,982,791 | TGGAGCTGGCACAAGAGTTC |
| TDP | 121,982,744 | 121,982,771 | CAGATGAAGACGGTTACAGTGTCCCTGG |
| TDR | 121,982,713 | 121,982,733 | AGCCGCACGACATCAGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCCATGAACACACACAAATC |
| SD | CGTGACCACTGTTTCTCGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 386 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 349 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10547A-C8 | VGB6 | C57BL/6NTac | |
| 10547A-D5 | VGB6 | C57BL/6NTac | |
| 10547A-D7 | VGB6 | C57BL/6NTac | |
| 10547A-A10 | VGB6 | C57BL/6NTac | |
| 10547A-C12 | VGB6 | C57BL/6NTac | |
