| Gene | Reg3a |
| Chromosome | 6 |
| Deletion Start | 78,381,091 |
| Deletion End | 78,383,604 |
| Deletion Size | 2,514 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,381,700 | 78,381,723 | GTCACAAAGGAAGCAGAAGCAAAC |
| TUP | 78,381,726 | 78,381,754 | TCAAGCAACAGGATCACAAGATGGAAAGC |
| TUR | 78,381,771 | 78,381,794 | GAGTGGGTGGCTACAATTAAGGTG |
| TDF | 78,383,254 | 78,383,273 | CTACTGTCAACCGTGGTCAC |
| TDP | 78,383,276 | 78,383,301 | TGGGAGTCTGACAGCAAGTTCAGGTA |
| TDR | 78,383,309 | 78,383,329 | GGGCAAAGATGGCTCTTCCTC |
PCR Primers
| Primer | Sequence |
| SU | GCACTAGCTATCACCCAGAC |
| SD | GCAAGATTGCAAAGCAGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 564 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 292 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10549A-E10 | VGB6 | C57BL/6NTac | |
| 10549A-E11 | VGB6 | C57BL/6NTac | |
| 10549B-G9 | VGB6 | C57BL/6NTac | |
