| Gene | Ccdc17 |
| Chromosome | 4 |
| Deletion Start | 116,596,769 |
| Deletion End | 116,627,427 |
| Deletion Size | 30,659 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,597,466 | 116,597,485 | GGGACCCAAGGGAGGATTAC |
| TUP | 116,597,486 | 116,597,507 | CCCGCCCATTCGACTTGGAGCG |
| TUR | 116,597,518 | 116,597,538 | GTTGGCCTCTTCCTTGAGTTC |
| TDF | 116,627,284 | 116,627,303 | CGGACTCTCCCTGAGGACTG |
| TDP | 116,627,308 | 116,627,329 | CAAGCTCGCCACAGTCCCGCTT |
| TDR | 116,627,331 | 116,627,350 | AGCTGGTTTCCGCTGACAAG |
PCR Primers
| Primer | Sequence |
| SU | AACTGACATTTTCACACCTGG |
| SD | CCAAACGGGAAGGGATACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 362 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 295 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10553A-A12 | VGB6 | C57BL/6NTac | |
| 10553A-B7 | VGB6 | C57BL/6NTac | |
| 10553A-D10 | VGB6 | C57BL/6NTac | |
| 10553B-C5 | VGB6 | C57BL/6NTac | |
