| Gene | Clec14a |
| Chromosome | 12 |
| Deletion Start | 58,268,831 |
| Deletion End | 58,267,444 |
| Deletion Size | 1,388 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,268,529 | 58,268,547 | GGAACGCAGCATCTCACAG |
| TUP | 58,268,501 | 58,268,528 | TGCACTCAGGAGAAAGAGCCTTTAAGGG |
| TUR | 58,268,481 | 58,268,500 | CGGGTGCAACCAGGAGAAAC |
| TDF | 58,267,553 | 58,267,572 | GACAGGGAAGGGAGCTTTGG |
| TDP | 58,267,526 | 58,267,551 | CTCACCAGGTGTGGAATGTGATGCCG |
| TDR | 58,267,489 | 58,267,511 | GTGCATTGTGTAGAACTGTGGTG |
PCR Primers
| Primer | Sequence |
| SU | AAAAGAGCCTCACTTGATCC |
| SD | GAATAGGAAAATGTCTCTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10554A-B6 | VGB6 | C57BL/6NTac | |
| 10554A-C8 | VGB6 | C57BL/6NTac | |
| 10554A-E7 | VGB6 | C57BL/6NTac | |
| 10554A-F10 | VGB6 | C57BL/6NTac | |
