| Gene | Zfp663 |
| Chromosome | 2 |
| Deletion Start | 165,359,976 |
| Deletion End | 165,352,327 |
| Deletion Size | 7,650 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 165,359,211 | 165,359,232 | ACAGAACAAGCTCCAGCCTAAG |
| TUP | 165,359,190 | 165,359,210 | TGTCCTCCGCAGCCAGAGCAG |
| TUR | 165,359,135 | 165,359,156 | GAAGCAAAGCAACAGGAGACAC |
| TDF | 165,353,017 | 165,353,037 | CCGGAGTGTGAGGGAAATGAG |
| TDP | 165,352,989 | 165,353,015 | AGCCGGACCATAGTGAAAACCAGAGCC |
| TDR | 165,352,923 | 165,352,942 | GGCTTGCAGTCTTGGGACAG |
PCR Primers
| Primer | Sequence |
| SU | ACTTGTTGACCTCTAGTTTG |
| SD | TGAGTTCTGCATCACATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 483 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | 362 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10560A-C9 | VGB6 | C57BL/6NTac | |
| 10560A-G9 | VGB6 | C57BL/6NTac | |
