| Gene | P2ry13 |
| Chromosome | 3 |
| Deletion Start | 59,210,352 |
| Deletion End | 59,209,316 |
| Deletion Size | 1,037 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,210,250 | 59,210,272 | GCTGTTCCCGGTTCTCTATACTG |
| TUP | 59,210,228 | 59,210,249 | TGGTCTTCCTGGCAGGCATCCT |
| TUR | 59,210,143 | 59,210,163 | TGCCACCAGAGTGTTCTTGAG |
| TDF | 59,209,645 | 59,209,664 | ACAAGCGGCTGGAGGTGAAG |
| TDP | 59,209,612 | 59,209,640 | TTATCGTCATGGCTGTCTTCTTTGTCTGC |
| TDR | 59,209,560 | 59,209,583 | TGGTGGTTTGACTGTAAGTGTATG |
PCR Primers
| Primer | Sequence |
| SU | CTATGTGCTCAAAAAAGCTC |
| SD | ATTTGCTGTATTACATGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 293 bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 306 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10561A-D8 | VGB6 | C57BL/6NTac | |
| 10561C-C4 | VGB6 | C57BL/6NTac | |
| 10561C-H6 | VGB6 | C57BL/6NTac | |
