| Gene | Ankrd1 |
| Chromosome | 19 |
| Deletion Start | 36,119,778 |
| Deletion End | 36,112,707 |
| Deletion Size | 7,072 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,119,542 | 36,119,561 | AAGGCAGGCACCTGGGATAC |
| TUP | 36,119,513 | 36,119,537 | AGCTATGCTCCAGACAGTGCACCGC |
| TUR | 36,119,494 | 36,119,512 | CAGCGACAGCCACCTTCAG |
| TDF | 36,113,665 | 36,113,685 | GGGTCCTCTTATCAGGTGCTG |
| TDP | 12,863,868 | 12,863,893 | ATGCTGTCTGTTTGGTGGTCCAGTGT |
| TDR | 36,113,568 | 36,113,589 | GCTGAAGAGAAGGGTGATCCTC |
PCR Primers
| Primer | Sequence |
| SU | CGGTGTGGTCACTGCCAAAG |
| SD | TCACTAGAGGATATTTTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 427 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | 268 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10563A-C5 | VGB6 | C57BL/6NTac | |
| 10563A-D6 | VGB6 | C57BL/6NTac | |
| 10563B-B6 | VGB6 | C57BL/6NTac | |
| 10563B-H9 | VGB6 | C57BL/6NTac | |
| 10563B-D3 | VGB6 | C57BL/6NTac | |
