| Gene | Gk2 |
| Chromosome | 5 |
| Deletion Start | 97,456,974 |
| Deletion End | 97,455,292 |
| Deletion Size | 1,683 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 97,456,529 | 97,456,553 | GCCTTCCTCTTAGCACTTACTTCAG |
| TUP | 97,456,495 | 97,456,518 | CTGCGGTGGATGCTGGACAACCTA |
| TUR | 97,456,455 | 97,456,476 | ACATGGCTCTGCCTTCCTCAAC |
| TDF | 97,455,694 | 97,455,713 | TGCAGGTCGATGGAGGAATG |
| TDP | 97,455,659 | 97,455,688 | CAACAGGATTCTTATGCAGCTACAGGCAGA |
| TDR | 97,455,615 | 97,455,634 | TCGTTTCAGGCATGACAGAC |
PCR Primers
| Primer | Sequence |
| SU | GAAATGGTATGGAGAGGTGAG |
| SD | TATAAAGGTAAATGTTGCATGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 275 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 371 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10566B-A10 | VGB6 | C57BL/6NTac | |
| 10566C-D11 | VGB6 | C57BL/6NTac | |
