| Gene | Cyp2r1 |
| Chromosome | 7 |
| Deletion Start | 114,562,954 |
| Deletion End | 114,550,077 |
| Deletion Size | 12,878 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,562,738 | 114,562,757 | GAAGCAGAGCCGGGTGTATG |
| TUP | 114,562,711 | 114,562,734 | AGGTAGGCCTCAACGCGGACCTTC |
| TUR | 114,562,625 | 114,562,644 | TCTGGCGTGGTATTGGTGAG |
| TDF | 114,550,310 | 114,550,331 | CCCAGGTTGGGCATGACATTAC |
| TDP | 114,550,284 | 114,550,306 | CCCAGCCCTACCTCATCTGTGCA |
| TDR | 114,550,263 | 114,550,283 | TTTGCACTTTCAGCGTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GTCCTCACCAAGAGCCAAG |
| SD | GTATGTGTGGGGGAATACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 343 bp | - | + | + |
| TUF | TUR | 133 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 245 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10568A-D10 | VGB6 | C57BL/6NTac | |
| 10568A-E11 | VGB6 | C57BL/6NTac | |
| 10568A-G3 | VGB6 | C57BL/6NTac | |
