10569   Suox     Status: ES cell colonies screened / QC positives

GeneSuox
Chromosome10
Deletion Start128,672,588
Deletion End128,670,496
Deletion Size2,093
Genome Build38
MGI IDMGI:2446117
NCBI Gene ID211389
BAC ID382O6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF128,672,097128,672,118GAGCATCGGTGTAGGGTAAGTG
TUP128,672,066128,672,093AAAGAGCTTCCATGCCGGAACAAAGGGA
TUR128,672,009128,672,030GGGAAATTAAGGGAGCAGGTTG
TDF128,670,983128,671,005GTGAGTGTGGAATCAGAGGAGAG
TDP128,670,954128,670,979TAGTCACTGGCAGAGGCGGGATTACA
TDR128,670,911128,670,933TCAAAGTTTACCGTGTCCCAGTC

PCR Primers

PrimerSequence
SUTCCTTTAGTCCCTTTGCCTG
SDTAGAGATGACCATAGGGTAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev353 bp-++
TUFTUR110 bp++-
TDFTDR95 bp++-
NeoFwdSD329 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10569B-F9VGB6C57BL/6NTac
10569B-C5VGB6C57BL/6NTac
10569B-C8VGB6C57BL/6NTac
10569B-E1VGB6C57BL/6NTac
10569B-E7VGB6C57BL/6NTac
10569B-F7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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