| Gene | Nhlrc1 |
| Chromosome | 13 |
| Deletion Start | 47,014,776 |
| Deletion End | 47,013,203 |
| Deletion Size | 1,574 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,014,174 | 47,014,193 | GCGACTGCTCCCTCAAAGTG |
| TUP | 47,014,143 | 47,014,171 | TGATTTCTTTGGCCAGATCAAGCTGGTTG |
| TUR | 47,014,059 | 47,014,079 | AAGTCCCTGCCTCTGCATCAG |
| TDF | 47,013,901 | 47,013,920 | TTGGGAGGACTGGCTGTAAC |
| TDP | 47,013,872 | 47,013,898 | CACAAGGGTGAAGGTGTTCAACTCCAC |
| TDR | 47,013,837 | 47,013,855 | GCCCGAAGCTATCCACCTG |
PCR Primers
| Primer | Sequence |
| SU | TGATTATCCCAGGTCTGCGC |
| SD | GCAGCCTTTGCCTTATAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 276 bp | - | + | + |
| TUF | TUR | 135 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 334 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10571D-E2 | VGB6 | C57BL/6NTac | |
| 10571E-H8 | VGB6 | C57BL/6NTac | |
