| Gene | Pex26 |
| Chromosome | 6 |
| Deletion Start | 121,184,250 |
| Deletion End | 121,193,628 |
| Deletion Size | 9,379 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,184,387 | 121,184,405 | GTCCGACCTGTTGGTGGTG |
| TUP | 121,184,406 | 121,184,427 | CACCTGGACTTCCATGCCGCAC |
| TUR | 121,184,430 | 121,184,447 | GGCTCGTTCGCAAGTCTC |
| TDF | 121,193,522 | 121,193,540 | TTCGCTGCCCTTCCTCTAC |
| TDP | 121,193,544 | 121,193,566 | CTGACCCAGCTCTTCCGCCGGAT |
| TDR | 121,193,570 | 121,193,588 | CCGAGAAAGTGTGGCCTTC |
PCR Primers
| Primer | Sequence |
| SU | GGGTTAGGCTGTCAGTGAG |
| SD | TGAGCTGTTGCAGTGTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 318 bp | - | + | + |
| TUF | TUR | 61 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 341 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10575B-C6 | VGB6 | C57BL/6NTac | |
| 10575B-D1 | VGB6 | C57BL/6NTac | |
