| Gene | Crybb3 |
| Chromosome | 5 |
| Deletion Start | 113,080,551 |
| Deletion End | 113,075,821 |
| Deletion Size | 4,731 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,079,658 | 113,079,678 | GCCTTTCTTTGCCTCAGTTTC |
| TUP | 113,079,630 | 113,079,657 | TCCATCCATAAGTTCCTGTGGCGTATGA |
| TUR | 113,079,608 | 113,079,626 | GCTGGCCTCCTTCCTGTTC |
| TDF | 113,076,682 | 113,076,701 | GTCTACGGCTCTCCCAACTC |
| TDP | 113,076,651 | 113,076,675 | CCCGCTCATTCTCGAGGTGATGGTG |
| TDR | 113,076,615 | 113,076,636 | TCGCTCCAGACCCAGATGATAG |
PCR Primers
| Primer | Sequence |
| SU | GCTGTCCTGAGGTCCAGTC |
| SD | GATGATCATATGGACAGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 479 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 292 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10576B-G4 | VGB6 | C57BL/6NTac | |
| 10576B-A12 | VGB6 | C57BL/6NTac | |
| 10576B-H8 | VGB6 | C57BL/6NTac | |
