| Gene | Tas2r138 |
| Chromosome | 6 |
| Deletion Start | 40,613,307 |
| Deletion End | 40,612,232 |
| Deletion Size | 1,076 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,613,088 | 40,613,107 | GGGCCTTCTGCTTCTGGATG |
| TUP | 40,613,062 | 40,613,084 | TTCAGCTCGCCTGCTTCCAGCAG |
| TUR | 40,613,040 | 40,613,059 | TGTGGCTCAGTGGGTCTTTC |
| TDF | 40,612,387 | 40,612,405 | GCTGAGGAGGGCCATAGAG |
| TDP | 40,612,358 | 40,612,385 | CCATGCTATTCTGGTTTCAAAGCAGGCA |
| TDR | 40,612,323 | 40,612,341 | TCCTGGGAGGAACCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | TCACAAGATACTGTAAAGTGAC |
| SD | AAGACTTCCCATGGGGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10577A-A12 | VGB6 | C57BL/6NTac | |
| 10577A-B6 | VGB6 | C57BL/6NTac | |
| 10577A-D3 | VGB6 | C57BL/6NTac | |
| 10577A-G9 | VGB6 | C57BL/6NTac | |
| 10577A-A11 | VGB6 | C57BL/6NTac | |
| 10577A-F9 | VGB6 | C57BL/6NTac | |
