| Gene | Car2 |
| Chromosome | 3 |
| Deletion Start | 14,886,756 |
| Deletion End | 14,900,133 |
| Deletion Size | 13,378 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,886,988 | 14,887,007 | GCAACACCCGACGACAATAC |
| TUP | 14,887,009 | 14,887,030 | TATCGGGACCACAGCCGGTCAC |
| TUR | 14,887,049 | 14,887,070 | AGTTACAACCCTTGCTCGATTC |
| TDF | 14,899,211 | 14,899,233 | GCATATCGTGGTCCACAAACAAG |
| TDP | 14,899,235 | 14,899,262 | TCTGGAGGTCTTGTGTACAGCTCAGAGC |
| TDR | 14,899,273 | 14,899,292 | GGGCATCTCAGAGGAGGAAC |
PCR Primers
| Primer | Sequence |
| SU | CACCTCCACGGTCTCCTC |
| SD | GCTGTGAAGAGAAGCAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 307 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10582A-D6 | VGB6 | C57BL/6NTac | |
| 10582A-E5 | VGB6 | C57BL/6NTac | |
| 10582A-G1 | VGB6 | C57BL/6NTac | |
| 10582A-B2 | VGB6 | C57BL/6NTac | |
| 10582A-G10 | VGB6 | C57BL/6NTac | |
| 10582A-H3 | VGB6 | C57BL/6NTac | |
