| Gene | Mogs |
| Chromosome | 6 |
| Deletion Start | 83,115,567 |
| Deletion End | 83,118,708 |
| Deletion Size | 3,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,116,245 | 83,116,262 | TCAGGTCAGGGCATCAGG |
| TUP | 83,116,264 | 83,116,288 | TCGTCTTTGCACAGCCCAGACTTCC |
| TUR | 83,116,317 | 83,116,339 | GGCTGTCAACCTCCATTTCATAC |
| TDF | 83,118,532 | 83,118,553 | GCTGCCAAACTCTACCATGAAC |
| TDP | 83,118,554 | 83,118,579 | TGCGGGCCAATGTAGTGAGAAACGTC |
| TDR | 83,118,602 | 83,118,625 | CACTGTACTGCTCCCACAGAAATC |
PCR Primers
| Primer | Sequence |
| SU | GCTCAGAATGCCATTGCTTC |
| SD | CATGCCTCCTAACCACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 353 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 380 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10584B-B9 | VGB6 | C57BL/6NTac | |
| 10584B-C6 | VGB6 | C57BL/6NTac | |
| 10584B-F11 | VGB6 | C57BL/6NTac | |
| 10584B-F8 | VGB6 | C57BL/6NTac | |
