| Gene | Pigm |
| Chromosome | 1 |
| Deletion Start | 172,376,699 |
| Deletion End | 172,377,967 |
| Deletion Size | 1,269 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 172,377,078 | 172,377,097 | ACTGCGTCTTCTGGCTTCTC |
| TUP | 172,377,099 | 172,377,121 | ACCCTTTGCCCATGGCGGTGTCC |
| TUR | 172,377,130 | 172,377,148 | GACGATGGAGTCCGCGTTG |
| TDF | 172,377,579 | 172,377,598 | CGTTCCTGCCACAGTTCATC |
| TDP | 172,377,599 | 172,377,621 | TTGCTGTCAGCCGCATCTTTCGC |
| TDR | 172,377,632 | 172,377,653 | GGAAACAGCAGAAGACAAGGTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGCGGACGCTAGACAGCC |
| SD | CTTCTCAACTGTGAAACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 398 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10589E-A5 | VGB6 | C57BL/6NTac | |
| 10589E-C2 | VGB6 | C57BL/6NTac | |
| 10589E-C11 | VGB6 | C57BL/6NTac | |
| 10589E-E12 | VGB6 | C57BL/6NTac | |
| 10589E-G1 | VGB6 | C57BL/6NTac | |
| 10589E-H10 | VGB6 | C57BL/6NTac | |
