10590   Bcs1l     Status: Germline transmission achieved

GeneBcs1l
Chromosome1
Deletion Start74,589,562
Deletion End74,592,073
Deletion Size2,512
Genome Build38
MGI IDMGI:1914071
NCBI Gene ID66821
BAC ID224I14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,589,74074,589,761CCTGGTTGCTTAGCTGGCTTAC
TUP74,589,76274,589,783CCGACACAGCACCCGCACTCAG
TUR74,589,81774,589,836GTGGAGATCCGGCCACTTTC
TDF74,591,56174,591,585GACTTCTCACATTCCTGTTATCCTC
TDP74,591,59074,591,619CCCTATAAAGTACCAAGGACTAGGCCGCCT
TDR74,591,62874,591,648CCAAAGCATTAAGCAGTCCAC

PCR Primers

PrimerSequence
SUGAGCAAAATGGATTGAAGAG
SDTGTGCCACAACACAGGGCAAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev373 bp-++
TUFTUR97 bp++-
TDFTDR88 bp++-
NeoFwdSD356 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10590A-B8VGB6C57BL/6NTac
10590A-D3VGB6C57BL/6NTac
10590A-H1VGB6C57BL/6NTac
10590B-E7VGB6C57BL/6NTac
10590B-A12VGB6C57BL/6NTac
10590B-A7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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