| Gene | Cldn16 |
| Chromosome | 16 |
| Deletion Start | 26,463,468 |
| Deletion End | 26,482,651 |
| Deletion Size | 19,184 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,464,137 | 26,464,155 | CTCAGGAACAGGCGTGGAC |
| TUP | 26,464,165 | 26,464,194 | CCACTCTAAACAACTGGTTACACAGCAGGA |
| TUR | 26,464,201 | 26,464,222 | ACCCAGTCACCTCCCTCATAAG |
| TDF | 26,482,548 | 26,482,569 | CCATGAGGAAGCCCTATTCAAC |
| TDP | 26,482,570 | 26,482,590 | TGCAGGTGTGTCCATGGCCAA |
| TDR | 26,482,609 | 26,482,632 | GCATACATTTTGGCTGTCTCTGTC |
PCR Primers
| Primer | Sequence |
| SU | TGCCACAGCCACAGGCCTCTG |
| SD | CTCCAAAGCAATAAAGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 474 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 337 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10591A-C7 | VGB6 | C57BL/6NTac | |
| 10591A-H8 | VGB6 | C57BL/6NTac | |
