| Gene | Gnptg |
| Chromosome | 17 |
| Deletion Start | 25,240,108 |
| Deletion End | 25,234,601 |
| Deletion Size | 5,508 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,239,922 | 25,239,942 | AAGGTGGTGGAGGAGCCTAAC |
| TUP | 25,239,897 | 25,239,921 | ACATTCGGGTGAGCGGATCACGGTC |
| TUR | 25,239,841 | 25,239,863 | GCCTATGGAACAGAGAAGGTCAG |
| TDF | 25,234,710 | 25,234,729 | CTGGCAGAAACCACTCACTC |
| TDP | 25,234,688 | 25,234,709 | TCAGCACCTGGGTCAGCAGCTC |
| TDR | 25,234,645 | 25,234,663 | CTGGGTCACTCCGCAGATG |
PCR Primers
| Primer | Sequence |
| SU | CTCCAAACTCACCACGCAGC |
| SD | GTTAAAGTTGTTAACATTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 374 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10592B-C8 | VGB6 | C57BL/6NTac | |
| 10592B-D2 | VGB6 | C57BL/6NTac | |
| 10592B-D6 | VGB6 | C57BL/6NTac | |
| 10592B-F2 | VGB6 | C57BL/6NTac | |
| 10592B-C2 | VGB6 | C57BL/6NTac | |
