| Gene | Zbtb46 |
| Chromosome | 2 |
| Deletion Start | 181,424,352 |
| Deletion End | 181,411,613 |
| Deletion Size | 12,740 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,423,459 | 181,423,480 | TGGCTCTCCAGTACCTTCATTC |
| TUP | 181,423,434 | 181,423,458 | CTACCCACATCGGGATGGCCTTTCA |
| TUR | 181,423,375 | 181,423,393 | TGACTTGGGCTGCCTACAC |
| TDF | 181,412,113 | 181,412,132 | TGGGCAGGTGGGATCAGTAG |
| TDP | 181,412,083 | 181,412,112 | CACAGTATACAAGGCTTCATGTTGCTCCGG |
| TDR | 181,412,046 | 181,412,068 | CGTCAGTCCAATCCAACCCTATG |
PCR Primers
| Primer | Sequence |
| SU | TGACCTCATACCTCCTTAGC |
| SD | TTTTAAGTTTACTAATTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10595A-A4 | VGB6 | C57BL/6NTac | |
| 10595A-F11 | VGB6 | C57BL/6NTac | |
| 10595A-F6 | VGB6 | C57BL/6NTac | |
| 10595A-F8 | VGB6 | C57BL/6NTac | |
| 10595A-E7 | VGB6 | C57BL/6NTac | |
| 10595A-E11 | VGB6 | C57BL/6NTac | |
