| Gene | Fstl3 |
| Chromosome | 10 |
| Deletion Start | 79,777,304 |
| Deletion End | 79,781,475 |
| Deletion Size | 4,172 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,777,688 | 79,777,708 | TGTAGGCCCTAGAGAGACATC |
| TUP | 79,777,710 | 79,777,731 | CTGGGTGGCTGGCCCTGCTAAT |
| TUR | 79,777,736 | 79,777,756 | TCCTGAAACCCTCTGCCTAAG |
| TDF | 79,781,067 | 79,781,086 | GACTCACAGGGAGGAGACAC |
| TDP | 79,781,087 | 79,781,112 | CATCCTGCACCTGACCCACCCTTTCT |
| TDR | 79,781,125 | 79,781,143 | CACTACCTGAGCGCAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CTGGACACCTGGAGGTGTCC |
| SD | TCTCCATGCAGCTGCCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 416 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10598A-A1 | VGB6 | C57BL/6NTac | |
| 10598A-B5 | VGB6 | C57BL/6NTac | |
| 10598A-B6 | VGB6 | C57BL/6NTac | |
| 10598A-H2 | VGB6 | C57BL/6NTac | |
| 10598A-D11 | VGB6 | C57BL/6NTac | |
| 10598A-G12 | VGB6 | C57BL/6NTac | |
