10601   Limd2     Status: ES cell colonies screened / QC one positive

GeneLimd2
Chromosome11
Deletion Start106,159,386
Deletion End106,158,674
Deletion Size713
Genome Build38
MGI IDMGI:1915053
NCBI Gene ID67803
BAC ID186F18
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,159,138106,159,155TCCTAAGGGTGGGTGTGG
TUP106,159,107106,159,129AAGGGTCGGCGCTAACCCGGCTT
TUR106,159,084106,159,106AGGAGCAGAGTACACTTAGCAAG
TDF106,158,847106,158,866CCAGACTGACCCAGATGTTC
TDP106,158,826106,158,845CTGCTTCCACAGCCTGGGCA
TDR106,158,807106,158,825CCGTGCATTGCAGCATAAC

PCR Primers

PrimerSequence
SUCTCGCTAACTTTGTTCTGTC
SDTGGGCTTGTGGGCCTCCCAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev433 bp-++
TUFTUR72 bp++-
TDFTDR60 bp++-
NeoFwdSD355 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10601A-B9VGB6C57BL/6NTac
10601A-H12VGB6C57BL/6NTac
10601A-H10VGB6C57BL/6NTac
10601A-H8VGB6C57BL/6NTac
10601A-G9VGB6C57BL/6NTac
10601A-H4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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