| Gene | Gnb3 |
| Chromosome | 6 |
| Deletion Start | 124,839,717 |
| Deletion End | 124,834,636 |
| Deletion Size | 5,082 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,839,393 | 124,839,411 | CCGCAGGATGCCAGGAAAG |
| TUP | 124,839,367 | 124,839,390 | TGTGCGGACATCACTCTGGCTGAG |
| TUR | 124,839,344 | 124,839,362 | CTCAGATGGGCAGAAGGTC |
| TDF | 124,835,489 | 124,835,510 | CCTCACCACTGATCCCTAACTG |
| TDP | 124,835,466 | 124,835,487 | TGCCTGACAGGAGCCTTGGGAA |
| TDR | 124,835,428 | 124,835,447 | GGGAACGCGGTCTTAGCTTC |
PCR Primers
| Primer | Sequence |
| SU | CGGAAACCTGGGAGAAGTC |
| SD | GTGGAGAGGAGAGGCCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10605A-A7 | VGB6 | C57BL/6NTac | |
| 10605A-G11 | VGB6 | C57BL/6NTac | |
| 10605A-E6 | VGB6 | C57BL/6NTac | |
| 10605A-A8 | VGB6 | C57BL/6NTac | |
| 10605A-B2 | VGB6 | C57BL/6NTac | |
| 10605A-B1 | VGB6 | C57BL/6NTac | |
