| Gene | Shroom1 |
| Chromosome | 11 |
| Deletion Start | 53,463,235 |
| Deletion End | 53,467,210 |
| Deletion Size | 3,976 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,463,965 | 53,463,983 | GCCCACTGGTGAGGACTTG |
| TUP | 53,463,989 | 53,464,016 | AGATTATTCCACGCAGGAGTTGCAGCGT |
| TUR | 53,464,027 | 53,464,047 | CCAGCAGCCCTTTGGACTTTG |
| TDF | 53,466,999 | 53,467,018 | GAGCGACCTCAGCCATCATC |
| TDP | 53,467,020 | 53,467,043 | CCTTTCTCCCAGACTGACCTGGGC |
| TDR | 53,467,102 | 53,467,122 | CAATGTTCCCACCTCCGCTAC |
PCR Primers
| Primer | Sequence |
| SU | ACTTGCGCGCATCCTATTGC |
| SD | TGTCTTTTGTGGAGGCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 383 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | 374 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10607A-G6 | VGB6 | C57BL/6NTac | |
| 10607A-G12 | VGB6 | C57BL/6NTac | |
| 10607B-H9 | VGB6 | C57BL/6NTac | |
| 10607B-D9 | VGB6 | C57BL/6NTac | |
| 10607B-D11 | VGB6 | C57BL/6NTac | |
| 10607B-G1 | VGB6 | C57BL/6NTac | |
