| Gene | Ltb4r2 |
| Chromosome | 14 |
| Deletion Start | 55,761,909 |
| Deletion End | 55,763,072 |
| Deletion Size | 1,164 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,762,608 | 55,762,628 | ATCGTGCTGGCCTTTGGCTTG |
| TUP | 55,762,630 | 55,762,649 | TCTGGGCTCCCTACCACGCG |
| TUR | 55,762,650 | 55,762,670 | CACCGCCTGTAGGAGATTGAC |
| TDF | 55,761,988 | 55,762,007 | CCGGCACTGCCTTTCTACTG |
| TDP | 55,762,015 | 55,762,037 | CGTTGCTGGGACTGCCAGGCAAT |
| TDR | 55,762,047 | 55,762,064 | GCCCGCTAAGCTCCATAC |
PCR Primers
| Primer | Sequence |
| SU | GTGCCTTGGTACCAGCGAC |
| SD | AGGAGCACACTTAAGCCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 353 bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 313 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10608A-A4 | VGB6 | C57BL/6NTac | |
| 10608A-A11 | VGB6 | C57BL/6NTac | |
| 10608A-H11 | VGB6 | C57BL/6NTac | |
| 10608A-B4 | VGB6 | C57BL/6NTac | |
| 10608A-H4 | VGB6 | C57BL/6NTac | |
| 10608A-D12 | VGB6 | C57BL/6NTac | |
