| Gene | 2810408M09Rik |
| Chromosome | 2 |
| Deletion Start | 165,493,299 |
| Deletion End | 165,491,423 |
| Deletion Size | 1,877 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 165,492,935 | 165,492,956 | GGGACAGGATCAGATGGCAATG |
| TUP | 165,492,906 | 165,492,932 | TAAGGACTCAAGGCGACAGTCAGGACG |
| TUR | 165,492,885 | 165,492,904 | CCCGCGAACCAAACAGAATG |
| TDF | 165,491,778 | 165,491,801 | GTAGTCTAAACCCTGTTCCAAAGC |
| TDP | 165,491,751 | 165,491,774 | CTGCCTGTGCATGAAGCTGGCCTT |
| TDR | 165,491,716 | 165,491,738 | AATGGACACACGTGGTTAGGGAA |
PCR Primers
| Primer | Sequence |
| SU | AAGCCGGAGCATCACCGTG |
| SD | CCACCCAGCTCTGCCTAGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10611D-G9 | VGB6 | C57BL/6NTac | |
| 10611E-H8 | VGB6 | C57BL/6NTac | |
| 10611E-A11 | VGB6 | C57BL/6NTac | |
| 10611E-A3 | VGB6 | C57BL/6NTac | |
