| Gene | Akr7a5 |
| Chromosome | 4 |
| Deletion Start | 139,310,741 |
| Deletion End | 139,318,253 |
| Deletion Size | 7,513 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,311,634 | 139,311,653 | GCCTTTGTGCCCATTCTCTG |
| TUP | 139,311,657 | 139,311,686 | CAGGTCTTACAATGGCAAACTCTGTAGCTC |
| TUR | 139,311,721 | 139,311,743 | TCCTCGTTGGACCTTTAACTCTC |
| TDF | 139,317,563 | 139,317,583 | CCCTCAGCTTCTGGAAGGAAC |
| TDP | 139,317,584 | 139,317,607 | ACCACTTTGAGGCCATCGCCCTGG |
| TDR | 139,317,624 | 139,317,642 | GTTGGTGCCATAGGTGGTC |
PCR Primers
| Primer | Sequence |
| SU | CAGGAGGGGACCAGCGCC |
| SD | CCTTTCTCATGGTCCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10612A-B7 | VGB6 | C57BL/6NTac | |
| 10612A-C11 | VGB6 | C57BL/6NTac | |
| 10612A-B2 | VGB6 | C57BL/6NTac | |
